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![88
IP 2007
produce 100.0 ml.To 2.0 ml of this solution add 50 ml ofwater,
10 ml of alizarine fluorine blue solution, 3 ml of a solution
containing 12 per cent w/v of sodium acetate and 6 per cent v/
v of glacial acetic acid, 10 ml of cerous nitrate solution and
sufficient water to produce 100.0 ml. Allow to stand in the
dark for 1 hour and measure the absorbance of a 4-cm layer of
the resulting solution at about 610 nm (2.4.7), using as the
blank a solution prepared in the same manner but using 2.0 ml
of water in place of the solution and beginning at the words
“To 2.0 ml...”. Calculate the flourine content from a reference
curve prepared by treating suitable aliquots of a solution of
sodium fluoride in the manner described above, beginning at
the words “add 50 ml of water.....”.
ForIodine
Burn the specified quantity of the substance under examination
in the prescribed manner using a mixture of 10 ml of water and
2 ml of 1 M sodium hydroxide as the absorbing liquid. When
the process is complete, add an excess (5 to 10 ml) of acetic
bromine solution and allow to stand for 2 minutes. Remove
the excess of bromine by the addition of formic acid (5 to 10
ml), rinse the sides of the flask with water and sweep out any
bromine vapour above the liquid with a current of air.Add 1 g
of potassium iodide and titrate with 0.02 M sodium
thiosulphate using starch solution, added towards the end
of the titration, as indicator.
1 ml of 0.02 M sodium thiosulphate is equivalent to 0.000423
g of I.
ForSulphur
Method I (in the absence of halogens and phosphorus) —
Burn the specified quantity of the substance under examination
in the prescribed manner using 10 ml of water and 0.1 ml
hydrogen peroxide solution (100 vol) as the absorbing liquid.
When the process is complete, cool the solution in ice for
about 15 minutes. Gently boil for 2 minutes, cool and add 50 ml
of ethanolic acetic-ammonia buffer pH 3.7. Titrate with 0.05
M barium perchlorate using 0.3 ml of alizarin red S solution
as indicator, until the solution becomes orange-pink in colour.
1 ml of 0.05 M barium perchlorate is equivalent to 0.001603 g
of S.
Method II (in the presence of halogens or phosphorus) —
Burn the specified quantity of the substance under examination
in the prescribed manner using 15 ml of water and 1 ml of
hydrogen peroxide solution (20 vol) as the absorbing liquid.
When the process is complete, boil the solution for 10 minutes,
cool and add 60 ml of ethanol (95 per cent). Titrate the solution
with 0.01 M barium perchlorate, using a 0.1 ml of 0.2 per cent
w/v solution of thoron and 0.1 ml of a 0.0125 per cent w/v
solution of methylene blue as indicator, until the yellow colour
changes to pink.
1 ml of 0.01 M barium perchlorate is equivalent to 0.0003206
g of S.
If the temperature at which the determinations prescribed under
Method I and II are performed differs from that at which the
barium perchlorate solution was standardised, the titrant
volumes are corrected by applying the expression
Vc = V [1 + 0.0008(t1 - t2)]
where, Vc = the corrected volume of titrant,
V = the volume of titrant used,
t1 = the temperature of the titrant during
standardisation,
t2 = the temperature of the titrant during the
determination.
2.3.35. Peroxide Value
The peroxide value is the number of milliequivalents of active
oxygen that expresses the amount of peroxide contained in
1000 g of the substance.
Method
Unless otherwise specified in the individual monograph, weigh
accurately about 5 g of the substance under examination,
transfer to a 250-ml glass-stoppered conical flask, add 30 ml of
a mixture of 3 volumes of glacial acetic acid and 2 volumes of
chloroform, swirl until dissolved and add 0.5 ml of saturated
potassium iodide solution.Allow to stand for exactly 1 minute,
withoccasionalshaking,add30mlofwaterandtitrategradually,
with continuous and vigorous shaking, with 0.01 M sodium
thiosulphate until the yellow colour almost disappears. Add
0.5 ml of starch solution and continue the titration, shaking
vigorously until the blue colour just disappears (a ml). Perform
ablankdeterminationomittingthesubstanceunderexamination
(b ml). The volume of 0.01 M sodium thiosulphate in the
blank determination must not exceed 0.1 ml.
Calculate the peroxide value from the expression
Peroxide value = 10 (a - b)/w
Where, w = weight, in g, of the substance.
3.3.36. Phenol in Vaccines andAntisera
Homogenise the preparation under examination. Dilute an
appropriate volume with water to give a solution containing
about 15 µg of phenol per ml.To 5.0 ml of the resulting solution
add 5 ml each of buffer solution pH 9.0, 4-aminophenazone
solution and potassium ferricyanide solution. Allow to stand
for 10 minutes and measure the absorbance of the resulting
solution at about 546 nm (2.4.7). Calculate the phenol content
from the absorbance obtained, using a calibration curve
2.3.35. PEROXIDE VALUE](/proxy?url=https%3A%2F%2Fimage.slidesharecdn.com%2Fi-140604070603-phpapp02%2F85%2FI-p-2007-volume-1-99-320.jpg)
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I.p. 2007 volume 1
This document provides information about the Indian Pharmacopoeia 2007 including its purpose, contents, and publication details. Some key points: - The Indian Pharmacopoeia Commission publishes the Indian Pharmacopoeia to establish quality standards for drugs in India in accordance with various laws. - This edition was prepared over two years with input from experts to update monographs, testing methods, and appendices based on advances in technology and international harmonization. - It is published in three volumes and will help regulate and improve drug quality in India. - Legal notices provide that the standards in the Indian Pharmacopoeia are legally enforceable and intended for licensing and inspection processes.
