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ESTIMATION OF SGOT

This document outlines the procedure for performing an SGOT test to determine aspartate aminotransferase (AST) levels in a blood sample. It describes that the SGOT test measures the oxidation of NADH to NAD, which is proportional to the AST activity in the sample. Instructions are provided for setting up the test with reagents, standard, test tubes, blood sample, and taking absorbance readings at intervals to calculate the AST activity level. Normal AST value ranges are listed for males and females, and increased levels are associated with hepatobiliary disease.

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• ABDUL BATEN
 Test tube  Test tube stand  Micropipette  Micro Tips  Blood sample  SGOT kit  Syringe  Vacutainer tube  Tourniquet
 SGOT (AST) catalyzes the transfer of amino group between L-Asparate and a-Ketoglutarate to form oxaloacetate and Glutamate.  The oxaloacetate formed reacts with NADH in the presence of Malate Dehydrogenase to form NAD.  The rate of oxidation of NADH to NAD is measured as a decrease in absorbance which is proportional to the SGOT(AST) activity in the sample.
L- Asparate + α- ketoglutarate L- Glutamate + oxaloacetate Oxaloacetate + NADH +H+ L- malate + NAD+
 WORKING REAGENT:- mix 4 parts of R1 + 1 part of R2  Pipette into clean dry test tubes labeled as Standard (S), and Test (T): T  Working Reagent:- 1000μl (1ml)  Specimen :- 100μl (0.10m) # Mix well and read the initial absorbance A after 1 min. and repeat 0 the absorbance reading after 1, 2 & 3 minutes at 37oC
 A/min X factor= AST activity( U/L)  Factor= 1746  NORMAL VALUES: (Serum)  Males : ≤37 U/L  Females: ≤ 31 U/L  CLINICAL SIGNIFICANCE: INCREASES: Increased levels are found in hepatobiliary disease.
ESTIMATION OF SGOT