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Dentin
Contour lines of Owen

• During development of dentin,
  variations in the metabolism of the
  organism cause variations in the
  amount of organic material deposited
  in the dentin, just as occurs in the
  enamel.
• Changes in the coloration of the
  dentin are called contour lines of
  Owen.
• The first dentin that is layed down (at
  the DEJ) forms the mantle layer while
  subsequent dentin forms the
  circumpulpal layer.
• In the crown of tooth, dentinal
  tubules from S-shaped primary             A, Contour line of Owen;
  curves.                                   B, Mantle layer of dentin;
                                            C, Circumpulpal layer of dentin;
                                            D, DEJ
Contour lines of Owen and Stria of
                Retzius
• The contour lines of
  Owen intercept the
  dentino-enamel
  junction and meet an
  accompanying Stria of
  Retzius that was
  formed at the same
  time.

                          A, Contour line of Owen;
                          B, Stria of Retzius
Lines of vonEbner
• Daily alterations in the
  formation of dentin
  produce imbrication lines
  of von Ebner that are
  approximately 5 µm in
  length, are seen as banding
  along the dentinal tubules
  and are comparable to the
  cross-striations in enamel.
• Arrow indicates the
  direction of the lines of
  von Ebner that are
  perpendicular to the
  dentinal tubules.
Dentinal tubules
• Each odontoblast has a long
  process that projects through the
  dentin to the DEJ.
• The hole or tube in the dentin
  through which this process
  projects is called the dentinal
  tubule.
• The course of the dentinal
  tubules in the crown of the tooth
  is S-shaped due to the crowding
  of the odontoblasts as they get
  squeezed into a smaller and
  smaller space within the pulp
  cavity.                             A, Primary curve of dentinal tubule;
• This curve is called a primary      B, DEJ; C, Mantle dentin;
  curve.                              D, Circumpulpal dentin
Odontoblasts in an erupted tooth
• As the dentin layer forms,
  the odontoblast become
  increasingly crowded as they
  are squeezed into a smaller
  area.
• The single layer of
  odontoblasts (as occurs in
  early development) has now
  changed to stacks of
  odontoblasts.
                                 A, Odontoblasts; B, Predentin;
                                 C, Pulp cavity;
                                 D, Dentinal tubules
Secondary curves
• During the deposition of
  dentin, the odontoblast
  makes slight undulations
  that creates wavy dentinal
  tubules.
• This waviness of the
  dentinal tubules is called
  secondary curves.
                               A, Secondary curve of dentinal tubule
Mantle dentin

• When viewed in polarized
  light, mantle dentin (red
  band), which is about 10
  µm wide, can be
  differentiated from
  circumpulpal dentin
  (purple with black
  dentinal tubules).
• This is due to a difference
  in the collagen fibers in
  mantle dentin.                A, Enamel;
                                B, Circumpulpal dentin;
                                C, Mantle dentin
Globular calcification of dentin
• Dentin is calcified after it
  is formed.
• The calcification begins in
  small spherical areas.
• These become larger and
  fuse with one another to
  form a calcification front
  (row of calcification
  sheres along the               A, Odontoblasts; B, Predentin;
                                 C, Calcification front;
  predentin edge).               D, Calcified dentin
Calcification pattern of dentin -
              decalcified section

• In mature dentin,
  globules of well-
  calcified dentin are
  apparent (arrow)
  along with areas of
  less calcified dentin
  (white areas).
                          A, Globule of well-calcified dentin
Interglobular dentin in globular layer -
             ground section
• Between the mantle and
  circumpulpal layers is a
  layer of dentin in which
  the calcified globules do
  not fuse evenly.
• This is called the globular
  layer.
• In a ground section of
  dentin, the less-calcified
  areas of dentin appear as
  irregularly shaped
  crescents called
  interglobular dentin.
                                A, Interglobular dentin
Globular layer and granular layer
• In the root of the tooth, the
  peripheral border of mantle
  dentin adjacent to the
  cementum has a granular
  appearance and is called the
  granular layer of Tomes.
• The granular layer is a less
  calcified layer like that of the
  globular layer.
• Generally, interglobular
  dentin is seen only in the
  crown, but in this specimen it
  extends into the root.             A, Circumpulpal layer; B, Globular layer;
                                     C, Mantle layer; D, Granular layer; E, CEJ;
                                     F, Enamel; G, Cementum
Dentinal tubules
• Dentinal tubules are the hollow tubes
  that run through the dentin in which
  are located the odontoblastic
  processes.
• During formation of dentin,
  intertubular dentin is layed down
  between the odontoblastic
  processes.
• Then a second layer of more
  mineralized dentin is added to "coat"
  the inside of the tubules.
• This layer of dentin is called
  peritubular dentin or intratubular
  dentin.
                                          A, Peritubular dentin;
• In this scanning electron micrograph,   B, Intertubular dentin;
  the peritubular dentin appears white.   C, Dentinal tubule
Dentinal tubular branches in root -
   transmitted and differential interface
           contrast microscopy
• Dentinal tubules
  generally have lateral
  branches where
  odontoblastic processes
  can communicate with
  one another.
• These branches, as seen
  here, are most numerous
                             A, Odontoblastic process;
  in the root of the tooth   B, Lateral branches of dentinal tubules
Terminal branching of dentinal
                    tubules
• Dentinal tubules are closely spaced
  but have a large diameter close to
  the pulp cavity.
• Dentinal tubules become more
  widely spaced in the dentin but
  have a narrower diameter as they
  approach the DEJ.
• At the DEJ, dentinal tubules branch.
• This causes an increased dentity of
  dentinal tubules in cross-sections of
  dentin in this region.
• In this micrograph, note the
  scalloped appearance of the DEJ.
                                          A, Dentinal tubule; B, DEJ;
• The peaks of the scallops point         C, Enamel; D, Enamel spindle
  toward the enamel.
Peritubular and intertubular dentin

• In this scanning
  micrograph of dentin,
  the dentinal tubules
  can be seen
  surrounded by a
  somewhat loosely
  packed intertubular
  dentin and a more
  dense peritubular
  dentin.                 A, Intertubular dentin;
                          B, Peritubular dentin;
                          C, Dentinal tubule
Forms of dentin
• Primary dentin, with straight tubules, is
  laid down before completion of the apical
  foramen.
• Regular secondary dentin is characterized
  by a slower rate of deposition and an
  abrupt change in the direction of the
  dentinal tubules.
• Tertiary or irregular secondary (also
  called irritation, reparative or reactive)
  dentin is laid down in response to an
  irritation or damage to the overlying
  dentin and/or enamel.
• This dentin has irregularly arranged and
  few dentinal tubules. With aging or severe
  damage, tertiary dentin can totally
  obliterate the pulp cavity.                  A, Primary dentin;
                                               B, Secondary (regular) dentin;
                                               C, Reactive dentin
Dead tracts and blind tracts
• When dentin is damaged,
  odontoblastic processes die or retract
  leaving empty dentinal tubules.
• Areas with empty dentinal tubules are
  called dead tracts and appear as dark
  areas in ground sections of tooth.
• With time, these dead tracts can
  become completely filled in mineral.
• This region is called blind tracts and
  appears white in sections of ground
  tooth.
• The dentin in blind tracts is called
  sclerotic dentin.
• The adaptive advantage of blind tracts
  is the sealing off of the dentinal
  tubules to prevent bacteria from       A, Dead tract;
  entering the pulp cavity.              B, Blind tract (containing sclerotic dentin)
This slide from:
Sharifa Abdulla Alhaj
This slide from:
Dalia.
Good
Luck
All
       Done by: Bedour Al-Arfaj.
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