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NME -BOTANY
-CULTIVATION OF OYSTER MUSHROOM
R. VELMURUGAN
Assistant Professor Of Botany
Saiva Bhanu Kshatriya College
Aruppukottai
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NME -BOTANY
-CULTIVATION OF OYSTER MUSHROOM
OYSTER MUSHROOM
Pleurotus ostreatus is commonly known as Oyster
Mushroom. The Fruit Bodies of this Mushroom are distinctly Shell
Shaped. It is the Second Most Cultivated Edible Mushroom next
to Button Mushroom. It Grows in 20-300C Temperature and their
Fruiting Bodies are Not Easy to Attack by any Pathogens and
Pests.
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NME -BOTANY
-CULTIVATION OF OYSTER MUSHROOM
Cultivation of White Oyster Mushroom
Steps in the Cultivation of Mushroom
⚫ Preparation of Spawn or Seed
⚫ Preparation of Substratum or Bed
⚫ Cultivation Method
⚫ Aftercare
⚫ Harvest and
⚫ Storage.
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CULTIVATION OF OYSTER MUSHROOM
-PREPARATION OF SPAWN or SEED
PREPARATION OF SPAWN or SEED
⚫ Wheat Seed with Fungal Mycelium called Spawn or Fungal Seeds.
⚫ Wash the Wheat Seeds in Water.
⚫ Dry the Seeds Under Shade.
⚫ Boil the Seeds in Water for 50 minutes.
⚫ Dry the Seeds in air by spreading on polythene sheet.
⚫ Coat the Seeds with 2% Calcium Carbonate and 1 % Chalk Powder to
adjust the pH around 7 and also keeping the Seeds in individuals.
⚫ Fill 250 g Seeds (Substrate) in Glass Bottle.
⚫ Close the Mouth by Cotton Plug for Good Aeration and also Prevents
the Entry of Microbes.
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CULTIVATION OF OYSTER MUSHROOM
-PREPARATION OF SPAWN or SEED
• Sterilize in Autoclave at 121ºC
for 30 minutes and then Cool.
• Inoculate the Pure Mycelium of
Pleurotus ostreatus into the
Bottle in Laminar Flow Chamber.
• Incubate at 25ºC for 10-12 Days
for Fungal Growth.
• During Incubation, the Mycelium
Grown in to Dense Mycelia on
Wheat Grains to form Spawn.
• The Spawn is used for the
Cultivation of Mushroom.
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CULTIVATION OF OYSTER MUSHROOM
-PREPARATION OF SUBSTRATUM
PREPARATION OF SUBSTRATUM
Organic Waste that used for Mushroom Cultivation is called Substratum
STEPS IN SUBSTRATUM PREPARATION
⚫ Cut the Paddy Straw or Sugarcane Bagasse in to 2.5 cm Size Pieces.
⚫ The Sugarcane Bagasse is Performed for the Best Production of Oyster
Mushroom when compared to Paddy Straw.
⚫ Soak in Water for 24 Hours.
⚫ Boil in Water for One Hour.
⚫ Dry Under the Shade the Pasteurized Substrates. But Maintains Moisture
Content up to 65 – 70%
⚫ Spray 10 % Calcium Carbonate Solution for Maintain the pH around 7.
⚫ Add Rice Bran, Gypsum and Molasses For High Yield.
⚫ Now, the Substrate Mixture is Ready for Mushroom Cultivation.
⚫ Use Polypropylene Bags (60 X 30 cm) for the Cultivation of Mushroom.
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CULTIVATION OF OYSTER MUSHROOM
- METHOD OF CULTIVATION
METHOD OF CULTIVATION
⚫ The Cylindrical Block System is Better for Oyster Mushroom
Cultivation.
⚫ Take the Substrate Mixture in Polypropylene Bag about 5 cm Height
as a First Layer.
⚫ Spread 50 gms of Spawn on the Substratum. This Process called
Spawning.
⚫ Make the Second Layer of Substratum about 10 cm Height.
⚫ Spread 50 gms of Spawn on the Substratum, mostly on the Periphery.
⚫ Repeat this Process for Several Times in the Same Manner.
⚫ Finally, the Spawn Covered with 5 cm Height of Substratum.
⚫ Make Many Holes on the Surface of the Bag for Watering, Good
Aeration and Reduce Temperature.
⚫ Tie the Mouth of Bag with Thread.
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CULTIVATION OF OYSTER MUSHROOM
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CULTIVATION OF OYSTER MUSHROOM
- AFTERCARE
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AFTERCARE
• Keep the Cylindrical Block Bed on the
Racks or in Hanging Position Under the
Cultivation Room or Shed.
• Sprinkling of Water Twice a Day with a
Very Fine Sprayer, keeping its Nozzle
Upside and Droplets Allowed to Come
Down Very Slowly and Maintain the
Temperature Between 20-300C and
Humidity about 80-85% .
CULTIVATION OF OYSTER MUSHROOM
- AFTERCARE
• Spawn Grows into Dense
Mycelium inside the Bag with in
10 -12 Days.
• After15 days, Remove the
Polypropylene Bag and Expose
the Bed in which Mycelium
starts to Develop into Knots
called Pin Head Stage.
• After 4-6 Days, the Pin Head
Stage Develops in to Shell
Shaped Oyster Mushroom.
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CULTIVATION OF OYSTER MUSHROOM
- HARVEST and STORAGE
HARVEST and STORAGE
⚫ After 21 Days of Spawning, starts to Harvest the Mushroom.
⚫ Harvest the Mushroom by Handpicked Method.
⚫ Harvest Minimum of about 500 to 700 kg of Fresh Oyster Mushroom
from One Ton of Dry Paddy Straw in 45-60 Days.
⚫ Remove the Dust Particles from Mushroom by Sterilized Forceps.
⚫ Fresh Mushrooms have a Shelf Life of 24-48 Hrs even at Room
Temperature
⚫ The Oyster Mushroom can be Easily to Dry and Stored in Powdered
Form for Future Use.
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